Sub-Cell GT Horizontal Electrophoresis Systems from Bio-Rad

Product Specs
ItemSub-Cell GT Horizontal Electrophoresis Systems
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1640302 / 1704402
Brand/TrademarkBio-Rad Laboratories, Life Science Group
Quantity1EA
Number of SamplesUp to 30 samples (15- and 20-wells)
Gel Dimensions15 x 15 cm gel tray

Specifications/Features

Bromophenol blue migration: ~3.0 cm/hr (at 75 V)

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Protocol for generating mutant zebrafish using CRISPR-Cas9 followed by quantitative evaluation of vascular formationSTAR ProtocDecember 2, 2023Juanjuan Luo, Chunjiao Lu, Maya Wang, Xiaojun YangP-1000 Microinjection mold for zebrafish,IBI Scientific,Cat# FM-200 Horizontal electrophoresis apparatus,Bio-Rad,Sub-Cell GT GStorm Gradient PCR,Thermo Fisher Scientific,Veriti Dx 100-mm Petri dish,Corning,Cat# 430167

(2) Population genomic analysis of an emerging pathogen Lonsdalea quercina affecting various species of oaks in western North AmericaScientific ReportsSeptember 8, 2023Olga Kozhar, Rachael A. Sitz, Reed Woyda, Lillian Legg, Jorge R. Ibarra Caballero, Ian S. Pearse, Zaid Abdo, Jane E. Stewartusing a MJ PTC-200 thermocycler (Bio-Rad Laboratories, Waltham, MA). PCR products were visualized via Sub-Cell GT Wide Mini electrophoresis system (BioRad, Hercules, CA, USA) that yielded a single band (286 kb in length)

(3) HDR-based CRISPR/Cas9-mediated Knockout of PD-L1 in C57BL/6 MiceBIO-PROTOCOLJuly 20, 2023Laura V. Heeb, Betül Taskoparan, Antonios Katsoulas, Michal Beffinger, Pierre-Alain Clavien, Sebastian Kobold, Anurag Gupta, Johannes Vom Berg(Vaudaux-eppendorf, catalog number: 5418/0005108)NanoDrop (DeNoVix DS-11 + spectrophotometer)Gel chamber (Bio-Rad, Sub-Cell GT)Machine to run gel (BioRad Power-Pac Basic)Machine to image gel (Quantum ST4, 1120 – Skylight Xpress)Flow cytometer

(4) Driver mutations of pancreatic cancer affect Ca2+ signaling and ATP productionFunction (Oxf)July 4, 2023Kinga B Stopa, Filip Łoziński, Agnieszka A Kusiak, Jacek Litewka, Daria Krzysztofik, Sylwester Mosiołek, Jan Morys, Paweł E Ferdek, Monika A Jakubowskaloading dye was then added to allow DNA detection in agarose gels. Casted gels were positioned in a Sub-Cell GT Horizontal Electrophoresis System (Bio-Rad, USA), covered with TAE buffer, loaded with 10 μL of DNA

(5) Triplet-primed PCR and Melting Curve Analysis for Rapid Molecular Screening of Spinocerebellar Ataxia Types 1, 2, and 3BIO-PROTOCOLJune 20, 2023Mulias Lian, Mingjue Zhao, Gui-Ping Phang, Indhu-Shree Rajan-Babu, Samuel S. Chongcentrifuge (Eppendorf, model number: 5810)Genetic Analyzer (Applied BiosystemsTM, model number: 3130xl)SUBCELL GT Horizontal Electrophoresis System (Bio-Rad, catalog number: A2-OK)NanoDropTM 1000 spectrophotometer

(6) Development of Cationic Lipid LAH4-L1 siRNA Complexes for Focused Ultrasound Enhanced Tumor UptakeMolecular PharmaceuticsMay 1, 2023Shahd Abuhelal, Miguel N. Centelles, Michael Wright, A. James Mason, Maya Thanouin water (Biotium, CA) and loaded into a 2% agarose gel. The gel was run for 40 min at 80 V using a Sub-Cell GT Agarose Gel Electrophoresis Systems (Bio-Rad laboratories, Watford, UK) and visualized under UV light. Stability

(7) Multidrug-Resistant Staphylococcus aureus Isolates in a Tertiary Care Hospital, Kingdom of BahrainCureusApril 7, 2023Abdullah AlSaleh, Mohammed Shahid, Eman Farid, Nermin Saeed, Khalid M Bindaynaextension (60s at 72°C), and a final extension for 4 min at 72°C. Gel electrophoresis was performed via Sub-Cell GT (Bio-Rad, CA, USA) on a 1.5% agarose gel and stained with ethidium bromide, afterward, the gel was visualized

(8) Safe-Shields: Basal and Anti-UV Protection of Human Keratinocytes by Redox-Active Cerium Oxide Nanoparticles Prevents UVB-Induced MutagenesisAntioxidants (Basel)March 20, 2023Francesca Corsi, Erika Di Meo, Daniela Lulli, Greta Deidda Tarquini, Francesco Capradossi, Emanuele Bruni, Andrea Pelliccia, Enrico Traversa, Elena Dellambra, Cristina Maria Failla, et al.conducted for 25 min using 0.81 V/cm and 300 mA or for 30 min using 1.00 V/cm, respectively, in a unit subcell GT system /15 × 25 cm system equipped with PowerPac™ HC High-Current Power Supply (Bio Rad Laboratories

(9) Identification and Expression Profile of CLE41/44-PXY-WOX Genes in Adult Trees Pinus sylvestris L. Trunk Tissues during Cambial ActivityPlants (Basel)February 13, 2023Natalia A. Galibina, Yulia L. Moshchenskaya, Tatiana V. Tarelkina, Kseniya M. Nikerova, Maxim A. Korzhenevskii, Aleksandra A. Serkova, Nikita V. Afoshin, Ludmila I. Semenova, Diana S. Ivanova, Elena N. Guljaeva, et al.reader SPECTROstar NANO, “BMG Labtech”, Ortenberg Germany) and by electrophoresis in 1% agarose gel (Sub-Cell GT Agarose Gel electrophoresis systems, Bio-Rad, USA). Before reverse transcription (RT), total RNA was

(10) Effectiveness of a Phage Cocktail as a Potential Biocontrol Agent against Saprophytic Bacteria in Ready-To-Eat Plant-Based FoodVirusesJanuary 6, 2023Michał Wójcicki, Olga Świder, Iwona Gientka, Stanisław Błażejak, Paulina Średnicka, Dziyana Shymialevich, Hanna Cieślak, Artur Wardaszka, Paulina Emanowicz, Barbara Sokołowska, et al.used (A&A Biotechnology, Gdansk, Poland). Electrophoresis was carried out at 80 V for 60 min using the Sub–Cell GT Horizontal Electrophoresis System (Bio–Rad, Madrid, Spain). The bands were visualized using the GeneFlash

(11) Protective Effect of Thyme and Chestnut Honeys Enriched with Bee Products against Benzo(a)pyrene-Induced DNA DamageInternational Journal of Environmental Research and Public HealthDecember 17, 2022Vanesa Sánchez-Martín, Ana I. Haza, Amaia Iriondo-DeHond, María Dolores del Castillo, Xavier F. Hospital, Manuela Fernández, Eva Hierro, Paloma Moralesdarkness for 40 min. Electrophoresis was performed in a cold storage room, in darkness, in a Bio-Rad subcell GT unit containing the same buffer, for 30 min at 25 V. After electrophoresis, the slides were neutralized

(12) Agarose Gel Electrophoresis-Based RAPD-PCR—An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological StudiesGelsNovember 22, 2022Tomasz Bogiel, Agnieszka Mikucka, Piotr Kanarekconcentrated Tris-Boric Acid-EDTA (TBE, Bio-Rad, Feldkirchen, Germany) running buffer in MINI SUB DNA CELL and SUB-CELL GT (Bio-Rad, Feldkirchen, Germany) devices at 9 V/cm within 90 min. Their images were captured and processed

(13) A microfluidic fully paper-based analytical device integrated with loop-mediated isothermal amplification and nano-biosensors for rapid, sensitive, and specific quantitative detection of infectious diseasesLab on a ChipNovember 22, 2022Hamed Tavakoli, Elisabeth Hirth, Man Luo, Sanjay Sharma Timilsina, Maowei Dou, Delfina C. Dominguez, XiuJun Lito measure the fluorescence intensity. LAMP products were also analyzed using gel electrophoresis (Sub-Cell GT, Bio-Rad, CA). For gel electrophoresis, 90 V was applied for 1 h in 1.5% agarose gel to resolve the

(14) Bacterial Pathogens in the Food Industry: Antibiotic Resistance and Virulence Factors of Salmonella enterica Strains Isolated from Food Chain LinksPathogensNovember 10, 2022Michał Wójcicki, Agnieszka Chmielarczyk, Olga Świder, Paulina Średnicka, Magdalena Strus, Tomasz Kasperski, Dziyana Shymialevich, Hanna Cieślak, Paulina Emanowicz, Monika Kowalczyk, et al.(A&A Biotechnology, Gdansk, Poland). Electrophoresis was carried out at 110 V for 60 min using the Sub-Cell GT Horizontal Electrophoresis System (Bio–Rad, Madrid, Spain). The bands were visualized using the GeneFlash

(15) Two heterozygous mutations in the calcium/ calmodulin‐dependent serine protein kinase gene ( CASK ) in cases with developmental disordersMolecular Genetics & Genomic MedicineNovember 1, 2022Kunfang Yang, Longlong Lin, Fang Yuan, Xiaoguang Li, Zhiping Liu, Xiaoping Lan, Yilin Wang, Yun Ren, Jiaoyan Li, Yucai Chenfurther analyzed by capillary gel electrophoresis using Image Lab software. Analysis was performed with Sub‐Cell GT Agarose Gel Electrophoresis Systems (Bio‐Rad Laboratories, Hercules, CA, USA). 3 RESULTS 3.1 Clinical

(16) Genotypic characterization of vancomycin-resistant Enterococcus causing urinary tract infection in northern IndiaIndian Journal of Medical ResearchSeptember 20, 2022Ayan Kumar Dudeja, AyanKumar Dudeja, Sunil Kohli, Pratima Raymin. PCR products were resolved on a 1.5 per cent agarose gel stained with ethidium bromide, using a Sub-Cell GT electrophoresis system (Bio-Rad, USA). A 100 bp DNA ladder (Vivantis Technologies Sdn. Bhd., Malaysia)

(17) Label free detection of SARS CoV-2 Receptor Binding Domain (RBD) protein by fabrication of gold nanorods deposited on electrochemical immunosensor (GDEI)Biosensors and BioelectronicsSeptember 15, 2022Deepshikha Shahdeo, Akanksha Roberts, G.J. Archana, Narlawar Sagar Shrikrishna, Subhasis Mahari, K. Nagamani, Sonu GandhiMassachusetts, USA). Agarose Gel Electrophoresis, SDS-PAGE and Western Blot analysis were performed using Sub-Cell GT electrophoretic system and Mini-PROTEAN Tetra Vertical Electrophoresis Cell respectively (Bio Rad laboratories,

(18) High-level gentamicin resistance mediated by Aac(6')-Ie-aph(2"")-Ia gene in Enterococcus species isolated from clinical samples in Northern IndiaIndian J PharmacolJuly 18, 2022Ayan Kumar Das, Mridu Dudeja, Sunil Kohli, Pratima Ray, Shyamasree Nandyrespectively. Electrophoresis of the amplified products was done on a 1.5% agarose gel with ethidium bromide on Sub-Cell GT electrophoresis apparatus (Bio-Rad, USA) at 100 Volts. 100-bp DNA ladder of 100-bp was used as standard

(19) Hobit and Blimp-1 regulate T(RM) abundance after LCMV infection by suppressing tissue exit pathways of T(RM) precursorsEuropean journal of immunologyJuly 1, 2022Parga-Vidal L, Taggenbrock RLRE, Beumer-Chuwonpad A, Aglmous H, Kragten NAM, Behr FM, Bovens AA, van Lier RAW, Stark R, van Gisbergen KPJM.material was loaded on a 2% agarose gel, separated by gel electrophoresis (100 V, Mupid‐One, BioRad Sub‐Cell GT) and imaged using the InGenius LHR Gel Imagining System (Syngene) to examine CRE recombinase‐driven

(20) WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damageNature communicationsJune 29, 2022Han SS, Wen KK, García-Rubio ML, Wold MS, Aguilera A, Niedzwiedz W, Vyas YM.Briefly, CometSlide (Trevigen, Gaithersburg, Md) plated with cells (2.5 ×105/mL) were placed in the Sub-Cell GT Agarose Gel Electrophoresis System (Bio-Rad Laboratories, Hercµles, Calif) for 45 min at 30 V in neutral

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